Clinical/Forensics


Clinical/Forensics Application Notes

Clinical and forensics laboratories looking for fast turnaround times will find that Gilson provides a variety of scientific applications to meet the throughput needs of the laboratory. From Solid Phase Extraction to Liquid Handling and optimization of manual techniques on automated platforms, Gilson provides a number of versatile solutions.

 

Normalization of DNA and RNA Samples with PIPETMAX® Normalization Assistant


 

KEYWORDS: PIPETMAX, Nucleic Acid Normalization, Normalization Workflow, Genomic DNA, cDNA, RNA, Isolation and Purification of Nucleic Acids, Normalization, Bovine DNA,

Download the Application Note


Streamlined Purification of Assay-ready Oligonucleotides by Automated HPLC

 

KEYWORDS: Oligonucleotides, qPCR, Next Generation Sequencing, Microarray Analysis, GX-271, Purification, TRILUTION LC

Download the Application Note

 

Preparation of Urine Samples for HPLC-MS/MS Detection of HNPAA Using the Gilson PIPETMAX® 268


 

KEYWORDS: PIPETMAX 268, HPLC-MS/MS, HNPAA, Metabolites. TNT, Sample Preparation

Download the Application Note

 


Automating the Promega GoTaq® PCR Core System II Reaction Using the Gilson PIPETMAX® 268


 

KEYWORDS: Promega GoTaq PCR Core System II, PIPETMAX 268, Plasmid DNA

Download the Application Note

 


High-Resolution Purification of Sheared Lambda Phage DNA Using a Gilson HPLC System


 

KEYWORDS: High-Performance Liquid Chromatography (HPLC), DNA Shearing, DNA Separation, Methylation, Lambda Phage, Gradient Pattern, Electrophoresis, Next-Generataion Sequencing (NGS)

Download the Application Note

 


Automated Patient Barcode Tracking and Sample Preparation Using the Gilson GX-281 Barcode Matching System


 

KEYWORDS: GX-281 Liquid Handler, Sample Tracking, Barcode Matching, Laboratory Information Management System (LIMS), Laboratory Informatics, Sample Preparation, Automation, Clinical Laboratory, Patient Samples

Download the Application Note

 


Selecting the Proper Pipetting Tools for Optimal PCR and qPCR in the Laboratory


 

KEYWORDS: PCR, qPCR, Pipetting, Biomolecule, Disposable Tips, Accuracy, PIPETMAN M, MICROMAN, PIPETMAN L, GmCLab

Download the Application Note

 


Efficient LC-MS/MS Method for Determination of delta9-Tetrahydrocannabinol in Human Plasma Using Solid Phase Extraction Clean-up


 

KEYWORDS: SPE, Tetrahydrocannabinol, Metabolites, Human Plasma, LC-MS/MS, LC-MS, GC-MS, MS

Download the Application Note

 


Methamphetamine and Amphetamine Drug Screening in Urine via Solid Phase Extraction


 

KEYWORDS: SPE, Urine Analysis, Drug Screening, mAMP, AMP

Download the Application Note

 


Automated NGS Library Prep: Ligation Efficiency of NxSeq® Sample Preparation Using The Gilson PIPETMAX®

 

KEYWORDS: Next Generation Sequencing, NGS, Sample Prep, DNA Library Prep, Automation, NxSeq, PIPETMAX

Download the Application Note

 


Determination of t, t-Muconic Acid in Urine via Automated Sample Preparation and HPLC 

KEYWORDS: Gilson GX-271 ASPEC with Dual Syringe Pump, TRILUTION LH v3.0 Liquid Handling Software, Solid Phase Extraction, SPE, Gilson GX-271 Liquid Handler, TRILUTION LC v2.1 Liquid Chromatography Software, human biomonitoring kit

Download the Application Note

 


Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually using Gilson PIPETMAN® M Multichannel, PIPETMAN L, and Automated using GX-271 ASPEC™ and GX-241 Liquid Handler

KEYWORDS: PIPETMAN® L, PIPETMAN M Multichannel, GX-241 Liquid Handler, GX-274 ASPEC™, Coomassie (Bradford) Protein Assay, Molecular Biology, Automation, Laboratory Equipment

Download the Application Note

 


An Undergraduate's Approach to Method Development: Chromatography Lessons Learned through Experimentation 

KEYWORDS: PLC 2020, Purification, Preparative Chromatography, Reversed Phase Chromatography, Method Development, Sparation Science, Pharmaceuticals

Download the Application Note

 


Manual Automation of Rapid N-Glycan Sample Preparation - Reliable Results from Error Free Sample Tracking and Accurate Pipetting

KEYWORDS: N-Glycan, Glycobiology, hlgG, Sample Cleanup, Purification, Glycoanalysis, Microchromatography

Download the Application Note

 


Automation of Solid Phase Extraction (SPE) Multi-method for GC/MS Analysis of Drugs of Abuse in Human Serum 

KEYWORDS: GX-271 ASPEC™, TRILUTION LH Liquid Handling Software, Solid Phase Extraction (SPE), Psychotropic drugs, Gas Chromatography Mass Spectrometry (GC/MS)

Download the Application Note

 


Improving Laboratory Efficiency & Flexibility with Automated Sample Preparation Systems - The Impact of the GX-281 Dual Z-Arm Weighing Station

KEYWORDS: Weighing Station, GX-281 Liquid Handler, Sample Preparation, Automated Systems, Bar Coding, Tube Gripper, TRILUTION LH v3.0 Liquid Handling Software

Download the Application Note

 


Automated Solid Phase Extraction (SPE) of Benzodiazepines from Urine using the Gilson GX-274 ASPEC™ 

KEYWORDS: Gilson GX-274 ASPEC™, TRILUTION® LH, Solid Phase Extraction (SPE), LC/MS-MS Analysis,Diazepam, Benzodiazepines, Urine, Pharmaceuticals

Download the Application Note

 


Automated Solid Phase Extraction of THC and Metabolites from Whole Blood using the Gilson GX-274 ASPEC™ 

KEYWORDS: Gilson GX-274 ASPEC™, TRILUTION® LH, Solid Phase Extraction (SPE), LC/MS-MS Analysis, Tetrahydrocannabinol (THC), Metabolites, Whole Blood

Download the Application Note

 


Automated Determination of VMA, HVA, 5-HIAA, in Urine Using the Gilson GX-274 and Gilson ASPEC™ XL

KEYWORDS: Gilson GX-274, TRILUTION® LH, Catecholamines, Urine, Vanillylmandelic acid (VMA), Homovanilic Acid (HVA), 5-hydroxyindoleacetic Acid (5-HIAA), Tumors, Diagnosis, Biosyntheses, Recipe

Download the Application Note

 


Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling

KEYWORDS: Automation, Biomarkers, Glycans, Glycomics, GX-274 ASPEC, Human Serum, Mass Profiling, Mass Spectrometry, MALDI-FTIR, MS, N-Glycan, N-linked Glycans, Serum, Solid Phase Extraction, SPE

Download the Application Note

 


An Automated Liquid Handling Strategy for Complex Matrices Based on Sample Viscosity 

KEYWORDS: Accuracy, Automated Liquid Handling, Beverages, Blood, GX-271, Liquid Handling, Liquid Transfer, Low Volume Dispensing, Optimization, Performance, Pipetting

Download the Application Note

 


Identification and Qualification of Illegal Drugs and Benzodiazepines in Human Plasma by LC/MS-MS Analysis after Solid Phase Extraction (SPE)

KEYWORDS: Amphetamines, Benzodizepines, Blood, Cocaine, Forensics, GX-271 ASPEC, Human Plasma LC/MS-MS, Opiates, Plasma, Solid Phase Extraction (SPE)

Download the Application Note

 


Automated Method for Dispensing Fruit Fly Food into Culture Vials and Bottles 

KEYWORDS: 215 Liquid Handler, Drosophila, Melanogaster, Fly Food, Fruit Fly, Genetics, Developmental Biology, Animal Models, Medical Research, Media, Culturing

Download the Application Note

 


Automated Fractionation System that Increases Recovery and Optimizes Precision of Solid Phase Extraction Methodology

KEYWORDS: Automated Fractionation, SPE, Serum, Plasma, Urine

Download the Application Note

 


Automated SPE Method for the Determination of Cotinine in Biological Fluid

KEYWORDS: Cotinine, Nicotine, GX-271 ASPEC/406 Dilutor, SPE

Download the Application Note

 


An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thiorizidine from Plasma

KEYWORDS: Neuroleptic drugs, SPE, SPE 215

Download the Application Note

 


An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen from Plasma

KEYWORDS: SPE, Ketoprofen, Ibuprofen, Prostaglandin

Download the Application Note

 


Accessing the E-Gel® 96 System via the Quad-Z 215 with Disposable Tips

KEYWORDS: QUAD-Z 215, Disposable Tips, E-Gel 96 gels, Agarose, DNA Electrophoresis, DNA Fragment

Download the Application Note

 


Quad-Z 215 with Disposable Tips: Implementing Use of ZipTips® for Purification of Bio Samples

KEYWORDS: QUAD-Z 215, Disposable Tips, ZipTips, Plasma, Biological Samples, Solid Phase Extraction (SPE), Purification, Concentration, Peptides, Proteins, Oligonucleotides

Download the Application Note

 


Reformatting Procedures Employing a Quad-Z 215 with Disposable Tips

KEYWORDS: QUAD Z-215, Disposable Tips, Microplate Reformatting, PCR Preparation, Protein Crystallography, Microplate Replication

Download the Application Note