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Application Notes
The Bradford Assay is a common universal protein concentration determination technique. In this app note, you'll learn how the automation of this qualitative application shows comparability between manual and automated results for preparing a standard curve and samples for quantification of protein in solution.
Mycotoxins are toxic compounds produced by fungi that are harmful to humans and animals. Patulin, found in rotting apples, is a compound that is regulated in many countries. Food safety testing for patulin is crucial to limit exposure; however, many sample cleanup methods do not remove closely related compounds, which can lead to false positive results.
This application note presents a new approach for the cleanup of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN® with the convenience of a DNA purification using MACHEREY-NAGEL’s proven NucleoMag® technology.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has been used to create useful animal models for studying complex diseases, as well as correcting or inducing disease states within a cell. This technical note describes how PLATEMASTER and EXTRACTMAN can be used in the rapid enrichment and clonal selection of CRISPR modified cells.
In this application note we examine the limits of quantification and detection, repeatability, reproducibility, and recovery in food safety analytical testing. Automation with the GX-271 ASPEC® system provides a reproducible and
reliable method for the isolation.
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.
Solid phase reversible immobilization (SPRI) beads are commonly used to clean up polymerase chain reaction (PCR) and DNA samples for downstream applications, such as sequencing and cloning. This application note demonstrates a flexible and reliable automated procedure for next-generation sequencing (NGS) library cleanup.
Gene expression studies are often used to gain insight into complex interactions between organisms, including those between plants and viruses. Real-time PCR (qPCR) is a tool frequently used in these studies given its wide dynamic range, sensitivity, and ease of automation. Learn how PIPETMAX can be used to automate qPCR sample prep in this informative app note.
This application note describes a high throughput sample preparation procedure for global glycan mass profiling.