As the drug resistance of malaria parasites increases, the development of innovative and effective antimalaria drugs is of high importance. In this app note, we describe how natural products provide a vast field of new lead structures that are effective in fighting the disease.

Vanillin is the major component of natural vanilla, which is one of the most widely used flavoring materials worldwide. In this app note, an ethanolic crude extract of vanilla is used to show the capacity of the PLC 2050 system to work with different types of chromatographic columns.

CPC is recognized as the preferred technology for the purification of high quality Δ-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) for the pharmaceutical and food supplement markets. The objective of this app note was to test the high selectivity of CPC and assess its capability to purify a set of key compounds (THC, CBD, CBN and CBG) and enrich several minor cannabinoids (CBDV, CBCV and CBC).

Ginger is widely known for its use in flavoring foods and beverages, but it is also commonly used for medicinal, perfumery, and esthetic purposes. This app note shows how CPC can produce a high-quality standard of [6]-gingerol without the use of silica-based chromatography methods, such as FLASH and prep HPLC.

Accurately quantify THC and metabolites for forensic purposes

Fermentation allows for the conversion of organic materials into relatively simple substances by using micro-organisms such as bacteria, Streptomyces, filamentous fungi, or yeast. In this app note, we demonstrate the added value of CPC at achieving an efficient separation from a fermentation broth in one step.

Artemisinin is a sesquiterpene lactone obtained from sweet wormwood, Artemisia Annua L. The extract of the leaves of Artemisia Annua L. is used in ancient Chinese herbal therapy for malarial fevers. Artemisinin has been identified as an active antimalarial compound. In this application note, the use of CPC coupled with a preparative liquid chromatography system equipped with UV-DAD and mass spectrometry (MS) detectors, illustrates the success of such technologies to isolate active compounds from complex mixtures.

The Coomassie (Bradford) assay is a well-established method for protein quantification that utilizes a commonly placed UV/Vis spectrophotometer. Streamlining the manual pipetting steps through automated liquid handling results in less data variability and improvements in workflow efficiencies.

Central Nervous System (CNS) depressants can introduce calm and induce sleep. Benzodiazepines are CNS depressants used to relieve anxiety, insomnia, and panic attacks. This application note discusses a simple SPE urine extraction before LC/MS-MS to confirm the presence of 15 spiked benzodiazepine analytes.

Forsythiaside A, a phenylethanoid glycoside, is one of the major active constituents extracted from the air-dried fruits of Forsythia suspensa and has been reported to possess a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities. This application note discusses a simple, fast, and cost-efficient method to prepare pure compounds using a centrifugal partition chromatography (CPC) system.