Peptide and protein purification are an intricate part of the proteomics workflow. Purification techniques include affinity, ion exchange, size exclusion, reverse phase chromatography, and gel filtration. The most popular means of detection is UV-VIS, but fluorescence and mass spectrometry can add another dimension. In addition to column-based techniques, proteins and peptides can also be purified using functionalized or magnetic beads. Gilson has a range of instruments to cover all these techniques.
Case Study: Helping Scale-up Peptide Purification in Drug Discovery
The Institute for Research and Innovation in Biomedicine (IRIB) in Normandy, France focuses on developing innovative technologies for diagnosing, treating, and preventing diseases. In this case study, we explore how the IRIB researchers used preparative HPLC in their peptide purification drug discovery applications to scale up their operations.
Desalting peptides prior to LC-MS (Liquid Chromatography-Mass Spectrometry) analysis is a crucial step in Proteomics sample preparation. This process removes salts and other contaminants that could interfere with the LC-MS analysis, ensuring higher sensitivity and better quality data. One of the most commonly used methods for desalting peptides is solid phase extraction (SPE), and this ASPEC® system provides the ultimate solution in versatility by combining flexible positive pressure SPE, precise liquid handling, large bed capacity, and optional direct injection in KC systems. It enables unattended processing of a high number of samples per day for peptide purification, making it an effective automated alternative to the StageTip-based manual methods.
The single quad VERITY 1920 Mass Spectrometer is ideal for laboratories seeking effective MS detection to isolate pure compounds. The versatile VERITY 1920 MS can utilize electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) ion sources, providing application flexibility. The detector can be combined with various Gilson Purification systems and selectively purifies active ingredients from small to large molecules and natural products from lab-to pilot-scale.
The VERITY® 271 LCMS system combines the greater selectivity of mass detection with the versatility of a mid-size capacity platform for semi-preparative to preparative HPLC purification. All configurations accommodate novel injection and fraction collection capabilities within a single platform, with the single quad VERITY® 1920 Mass Spectrometer equipped with electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) ion source. This is the ideal solution for peptide purification and laboratories seeking effective MS detection to isolate pure compounds from synthetic and natural products from lab to pilot scale.
Designed for semi-preparative to preparative purification, the reliable and versatile VERITY 1741 UV-VIS Detector is coupled with the power of multiple-wavelength scanning detection for more significant information and data gathering at a lower cost of ownership. With the ability to scan multiple different wavelengths simultaneously, the VERITY 1741 Detector provides the detection specificity required for a wide array of purification applications.
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