In this technical note, we highlight how our software and products help you add extra dimensions to your fraction collection possibilities allowing you to set conditions according to specific requirements for synthetic compound purification.
Josh Martin, a graduate research assistant and Ph.D. candidate with the McCardle Laboratory for Cancer Research, discusses how EXTRACTMAN helps to overcome limitations of the immunoprecipitation techniques that aim to identify and isolate binding partners of proteins.
Protein-protein interactions play an ever important role in oncogenesis. In this video, Dr. Richard Burgess, Professor Emeritus of Oncology at the University of Wisconsin, discusses approaches to characterize weak protein interactions that are targeted by cancer researchers.
Kinase profiling is a necessary process to confirm inhibitor selectivity and assess off-target activity. The arduous task of optimizing kinase reactions and maintaining a library of enzymes often results in the outsourcing of kinase profiling activities. This poster breaks down a flexible workflow for the automation of bioluminescent kinase selectivity profiling.
Liquid chromatography coupled with mass spectrometry (LCMS) is widely used in both analytical and preparative chemistry applications. This technical note describes LCMS purification of selected compounds from a mixture of five peptides, using conditional fraction collection logic to collect only the peaks that met the criteria set for both UV signal and MS signal.
Automation of the Kinase Selectivity Profiling System with PIPETMAX, in conjunction with automated detection and data analysis methods, offers a streamlined workflow for single-point and dose-response evaluation of potential cancer therapies. Learn more in this application note.
Automation of the Coomassie Bradford Protein Assay Using a Multiple Channel Bench Top Pipetting System
The Bradford Assay is a common universal protein concentration determination technique. In this app note, you'll learn how the automation of this qualitative application shows comparability between manual and automated results for preparing a standard curve and samples for quantification of protein in solution.
RNA-binding proteins (RBPs) are involved in many key cellular processes, but identification of the native binding partners (target RNAs) can be challenging. In this application note, we show that EXTRACTMAN can be used to efficiently and specifically enrich endogenously expressed ribonucleoprotein complexes.
In this app note, superparamagnetic Protein G particles from three suppliers were tested with a simple immunoprecipitation protocol to evaluate their performance on EXTRACTMAN.
EXTRACTMAN® Delivers Expanded Analyte Isolation of Weakly- or Transiently-Bound Species through ESP™
This application note describes a comparison of a conventional Co-IP protocol with an EXTRACTMAN® protocol using ESP technology. While the conventional Co-IP protocol did not pull down the protein-protein complex, EXTRACTMAN did, demonstrating the utility of ESP technology and EXTRACTMAN.