Next Generation Sequencing (NGS)
Reliable and reproducible library preparation is critical for successful next generation sequencing (NGS). In this poster, We compare sequencing results for E. coli whole genome sequencing libraries prepared with the Illumina Nextera XT kit, with liquid handling carried out either manually or automated with PIPETMAX.
Next generation sequencing (NGS) became essential in routine HLA typing due to its efficiency in the context of resolution, costs, and throughput. To understand the required pipetting steps during NGS library preparation, we integrated the manual 96-channel pipetting system PLATEMASTER into our NGS workflow.
This application note describes the setup and execution of automation of the Illumina® Nextera® XT DNA Library Preparation Kit using the PIPETMAX automated liquid handler. Five different scripts have been created and are described in detail in this document.
Solid phase reversible immobilization (SPRI) beads are commonly used to clean up polymerase chain reaction (PCR) and DNA samples for downstream applications, such as sequencing and cloning. This application note demonstrates a flexible and reliable automated procedure for next-generation sequencing (NGS) library cleanup.
Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.
Automated NGS Library Prep Ligation Efficiency of NxSeq Sample Preparation Using the Gilson PIPETMAX® 268
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.