DNA / RNA Normalization
In this study, functional corn RNA was quantitated by RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System after two different extraction methods. To enhance the pipetting accuracy and consistency an automated pipetting assistant, PIPETMAX was used to reduce pipetting errors inherent in manual methods.
Normalization of the concentration of nucleic acid samples is a crucial yet tedious process that requires constant readjustment of the volume of the pipetting device. PIPETMAX® Normalization Assistant automates this process: calculations are carried out by the software, eliminating arithmetic errors, and pipetting volumes are adjusted automatically - learn more in this app note.
Quantitative real time PCR, often coupled with reverse transcription, is a very sensitive technique that can be used for many applications including gene expression analysis. PIPETMAX Normalization Assistant delivers an easy-to-use software and hardware solution to automate normalization of sample concentration without compromising RNA integrity.
This application note describes the setup and execution of automation of the Illumina® Nextera® XT DNA Library Preparation Kit using the PIPETMAX automated liquid handler. Five different scripts have been created and are described in detail in this document.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has been used to create useful animal models for studying complex diseases, as well as correcting or inducing disease states within a cell. This technical note describes how PLATEMASTER and EXTRACTMAN can be used in the rapid enrichment and clonal selection of CRISPR modified cells.