Liquid chromatography (LC) is the standard method used for purifying products in the discovery stages, as it is cost-effective, relatively simple to perform, and reliable. However, obtaining high purity and yield using this approach often comes at the cost of throughput, as purification runs must be lengthened to achieve greater separation between components. This article explores how users can enhance fraction collection efficiency and streamline the purification process by using the latest advances.

How do you know you’re pipetting the volume you intend? Would you trust the results you’re getting if you didn’t know your pipettes were calibrated properly? Fortunately, there are standards that pipette manufacturers and calibration services follow so that you know you’re pipetting the correct volumes. Read more here.

When choosing a high performance liquid chromatography (HPLC) system, it's important to consider how your future needs may evolve from your current needs. In this article, you’ll learn about the advantages of modular HPLC systems when it comes to current experimental needs and how the ability to swap out HPLC components safeguards the future of your system and your research.

Preparative chromatography, a method used to separate and purify target compounds from complex mixtures, plays a crucial role in the chemical and pharmaceutical industries. In either industry, preparative chromatography has roles in the early stages of drug development, pilot studies, and process development. In this article, you'll learn how we provide support throughout this process and enable our customers and partners to achieve their purification goals from the milligrams to the kilogram scale.

Quantitative polymerase chain reaction (qPCR) is a powerful and highly sensitive method of amplifying genetic material, with applications ranging from diagnostics and forensics to research. However, ensuring the accuracy and reliability of your qPCR experiments can be challenging — even the smallest amount of contamination or failure to adhere strictly to liquid handling best practices can substantially impact your results. So, what can you do to maximize your chances of delivering highly accurate qPCR results, time after time? In this article, we share our top 5 tips for qPCR success.

Per- and polyfluoroalkyl substances (PFAS), or “forever chemicals”, are highly stable materials used in a variety of household products, industries, and processes. These chemicals can pose several health risks to living organisms and contaminate the environment. In this article, we elaborate on the above along with how automated solid phase extraction can be used to isolate PFAS from numerous matrices including water, soil, air (dust), and food such as seafood and eggs.

Sample integrity is vital for accurate, trustworthy results and it can be affected by all stages of liquid handling, from pipetting to transport. Taking actions to improve pipetting and pipette storage, the lab environment, and sample storage and transport can protect your sample integrity and give you trustworthy results – learn more here.

Synthetic oligonucleotides (oligos) are central to many cutting-edge biochemical applications across research and therapeutics. While solid-phase synthesis typically yields oligos with relatively high purity, they usually need further purification to avoid unintended side-reactions and interferences in downstream applications. Preparative HPLC is the preferred method for small to medium length oligos (10-60 bp). To increase productivity and maximize the profitability of your oligo research and development, finding the optimal LC purification platform is crucial.

Switching from a manual to an automated process not only enables significantly more effective use of personnel time, but also removes operator error for reproducible results and increases the accuracy and precision of assays. In this article, we delve into the challenges that can arise during this transition and explain how Gilson's solutions and services can ease the process of transferring your manual methods to an automated system.

In this case study, learn how one molecular biology and genomics lab is saving time, improving data traceability, and standardizing protocols by using the TRACKMAN Connected pipetting system.