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Application Notes
Since 1970, glyphosate has been used as a systemic, non-selective herbicide and crop desiccant on farms, in fields, and in public and residential areas. This application note describes the rapid purification of glyphosate, aminomethylphosphonic acid (AMPA), and glufosinate from red wine using AFFINIMIP® SPE Glyphosate.
Per- and poly-fluoroalkyl substances (PFAs), sometimes referred to as perfluorinated compounds, are a family of molecules consisting of varying lengths of fluorocarbon chains with a functional group such as carboxylic or sulfonic acid attached. This application note describes the automation of the isolation of 10 PFAs from tap water prior to their analysis by LC/MS.
Δ9-tetrahydrocannabinol and its metabolites are essential for drug testing. GC/MS is often used for quantification of Δ9-tetrahydrocannabinol, however, it is time consuming. Rapid methods that maintain the sensitivity and selectivity of GC/MS are desirable - learn more in this application note.
Deoxynivalenol (DON) is a mycotoxin that occurs predominantly in grains such as wheat, barley, oats, rye, and maize and consumption is associated with outbreaks of gastroenteritis in humans. Automated methods are well-suited for quantification of toxins because they are accurate, reduce the time required for laboratory analysis, and reduce human errors - learn more in this application note.
In this application note, three glucocorticoids and three nonsteroidal anti-inflammatory drugs were detected simultaneously from horse urine using the Gilson GX-274 ASPEC® system with two step extractions and LC/MS/MS selected reaction monitoring (SRM).
This application note describes a comparison of a conventional Co-IP protocol with an EXTRACTMAN® protocol using ESP technology. While the conventional Co-IP protocol did not pull down the protein-protein complex, EXTRACTMAN did, demonstrating the utility of ESP technology and EXTRACTMAN.
In this app note, superparamagnetic Protein G particles from three suppliers were tested with a simple immunoprecipitation protocol to evaluate their performance on EXTRACTMAN.
EXTRACTMAN® improved isolation speed nine-fold and produced equivalent or better yields of purified DNA compared to tube-based isolation methods. In this application note, four DNA samples from two different organisms (mammalian and plant) were processed in parallel with no cross-contamination.
RNA-binding proteins (RBPs) are involved in many key cellular processes, but identification of the native binding partners (target RNAs) can be challenging. In this application note, we show that EXTRACTMAN can be used to efficiently and specifically enrich endogenously expressed ribonucleoprotein complexes.
This application note presents a new approach for the cleanup of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN® with the convenience of a DNA purification using MACHEREY-NAGEL’s proven NucleoMag® technology.