A Comparative Study Between CPC and Flash Chromatography for Natural Product Purification

Piperine, an alkaloid that gives black pepper its pungent taste (Piper nigrum), is also associated with several bioactivities and can be used as a spray in personal defense devices. The preparative separation and purification of piperine is usually performed by conventional methods. Among those methods are centrifugal partition chromatography (CPC) and flash chromatography. In this study, a crude extract of black pepper is used to purify piperine and to compare the performance of CPC versus flash chromatography - read more about the methods and results in this application note..